Steps and Examples
We create a compact way to describe a set of instruction
Flags
| BIT | PARAM_STATUS | COMMENT |
|---|---|---|
| 0 | PID | enable/disable heating |
| 1 | Stepper | enable/disable agitation control |
| 2 | OUTPUT 1 | enable/disable output 1 |
| 3 | OUTPUT 2 | enable/disable output 2 |
| 4 | OUTPUT 3 | enable/disable output 3 |
| 5 | OUTPUT 4 | enable/disable output 4 |
Actions
0 xxx x yyy yyyy yyyy
| xxxx | Action | |
|---|---|---|
| 0 | 0000 | Do nothing |
| 1 | 0001 | Wait in minutes |
| 2 | 0010 | Wait in hours |
| 3 | 0011 | Wait for weight reduction to yy% of maximum weight |
| 4 | 0100 | Wait for weight increase to yy% of maximum weight |
| 5 | 0101 | Wait for temperature change (continue if delta < yy [°C/100]) |
| 8 | 1000 | Set all the flags |
Change parameters
1 xxx x yyy yyyy yyyy
- xxxx the parameter (0 -> 15)
- 0: Target temperature (in °C)
- yyy yyyy yyyy: the new value (0 to 2047)
Examples
Example 1
Using two peristaltic pumps to remove bacteria and add fresh media:
| Step | Instruction | Explanation |
|---|---|---|
| 0 | 0 1000 000 0000 0011 (16387) | Stepper: on, PID: on |
| 1 | 0 0010 000 0001 1000 (4120) | Wait 1 day (24 h) |
| 2 | 0 1000 000 0000 0000 (16384) | Stepper: off, PID: off |
| 3 | 0 0001 000 0001 1110 (2078) | Wait 30 min |
| 4 | 0 1000 000 0000 0100 (16388) | OUT1: on |
| 5 | 0 0011 000 0001 1110 (6174) | Weight reduction 30 % |
| 6 | 0 1000 000 0000 1011 (16395) | OUT2: on, Stepper: on, PID: on |
| 7 | 0 0100 000 0110 0100 (8292) | Weight increase 100 % |
| 8-15 | 0 0000 000 0000 0000 (0) | Do nothing |
Example 2
Changing temperature and turning on / off a light (on IO3). We simulate a day / night cycle:
| Step | Instruction | Explanation |
|---|---|---|
| 0 | 0 1000 000 0001 0011 (16403) | IO3: on, Stepper: on, PID: on |
| 1 | 1 0000 000 0010 1000 (32808) | Set target temperature to 40 °C |
| 2 | 0 0010 000 0000 1100 (4108) | Wait 12 h |
| 3 | 0 1000 000 0000 0011 (16387) | IO3: off, Stepper: on, PID: on |
| 4 | 1 0000 000 0001 1110 (32798) | Set target temperature to 30 °C |
| 5 | 0 0010 000 0000 1100 (4108) | Wait 12 h |
| 6-15 | 0 0000 000 0000 0000 (0) | Do nothing |
Example 3
Run bioreactor with temperature control, agitation control, and adding fresh media in steps of 25% of maximum weight every 30 minutes; when it obtains 100% weight it continues for 16 hours.
| Step | Instruction | Explanation |
|---|---|---|
| 0 | 0 1000 000 0000 0111 (16391) | IO1: on, Stepper: on, PID: on |
| 1 | 0 0100 000 0001 1001 (8217) | Weight increase until 25 % |
| 2 | 0 1000 000 0000 0011 (16387) | IO1: off, Stepper: on, PID: on |
| 3 | 0 0001 000 0001 1110 (2078) | Wait 30 min |
| 4 | 0 1000 000 0000 0111 (16391) | IO1: on, Stepper: on, PID: on |
| 5 | 0 0100 000 0011 0010 (8242) | Weight increase until 50 % |
| 6 | 0 1000 000 0000 0011 (16387) | IO1: off, Stepper: on, PID: on |
| 7 | 0 0001 000 0001 1110 (2078) | Wait 30 min |
| 8 | 0 1000 000 0000 0111 (16391) | IO1: on, Stepper: on, PID: on |
| 9 | 0 0100 000 0100 1011 (8267) | Weight increase until 75 % |
| 10 | 0 1000 000 0000 0011 (16387) | IO1: off, Stepper: on, PID: on |
| 11 | 0 0001 000 0001 1110 (2078) | Wait 30 min |
| 12 | 0 1000 000 0000 0111 (16391) | IO1: on, Stepper: on, PID: on |
| 13 | 0 0100 000 0110 0100 (8292) | Weight increase until 75 % |
| 14 | 0 1000 000 0000 0011 (16387) | IO1: off, Stepper: on, PID: on |
| 15 | 0 0010 000 0001 0000 (4112) | Wait 16 h |